Publications
2025
Wang Y, Nakagawa M*, Luo C, Kanda R, Matsushima Y, Nishiura A, Honda Y*. Cellular senescence of RANKL(+) osteoblasts and Th17 cells in severe periodontitis with occlusal trauma. J Prosthodont Res. 2025. In press.
(Aging cell/regenerative medicine research) This research attempted to clarify the mechanism of periodontitis associated with occlusal trauma. In a rat model, it was demonstrated that stress-induced premature aging (osteoblasts and Th17 cells) cells are involved in the aggravation of the disease, and that it may be possible to prevent this with a senescent cell removal drug (senolytic). This knowledge will lead to the development of next-generation periodontal disease treatments.
2024
Wang X, Zhou Y, Luo C, Zhao J, Ji Y, Wang Z, Zheng P, Li D, Shi Y, Nishiura A, Matsumoto N, Honda Y*, Xu B, Huang F. Senolytics ameliorate the failure of bone regeneration through the cell senescence-related inflammatory signalling pathway. Biomed Pharmacother. 2024; 175: 116606. (IF=7.4)
(Aging stem cell research) Dental pulp stem cells undergo cellular aging when cultured for a long period of time. However, the details of this cellular aging phenomenon have not yet been elucidated. We carefully obtained, analyzed, and reported basic knowledge for the future application of dental pulp stem cells in regenerative medicine and for elucidating stem cell aging.
(Aged cells/regenerative medicine research) If bacterial components are mixed in, it will greatly worsen the outcome of surgery. We have demonstrated in a rat model that the stress caused by lipopolysaccharide (LPS) contamination during calcium phosphate implantation causes stress-induced premature senescent cells to appear, and that bone regeneration can be restored by controlling these cells. This is basic knowledge that will lead to the realization of safe regenerative medicine.
Zheng S, Nakagawa M, Gong Y, Matsushima Y, Sasayama S, Baba S, Honda Y*. Temporal changes toward cellular senescence in rat dental pulp stem cells induced by long-term in vitro culture. Appl Sci. 2024; 14: 11376.
Deng Z, Iwasaki K*, Peng Y, Honda Y. Mesenchymal stem cell extract promotes skin wound healing. Int J Mol Sci. 2024; 25: 13745.
(Regenerative medicine research) This is a study that attempted to apply mesenchymal stem cell extracts to regenerative medicine. With the cooperation of a professor in the field of advanced dentistry, we reported that the extract accelerates the healing of skin injuries. This result will lead to the development of effective treatments for skin diseases that occur in the fields of dental oral surgery and plastic surgery.
2023
Zhou Y, Nishiura A*, Morikuni H, Deng W, Tsujibayashi T, Momota Y, Azetsu Y, Takami M, Honda Y*, Matsumoto N. RANKL+ senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolytics. IJOS 2023; 15: 20.
(Senescent cell research) There is still no clear method for preventing or treating root resorption that occurs during orthodontic treatment. Using rats, we were the first in the world to report that stress-induced premature senescent cells are involved in the mechanism of the disease, and that it can be prevented with a senescent cell removal drug (senolytic). (This was introduced as a featured article in IJOS (IF=14.9), a journal published by Nature Group, which has the highest impact factor in dentistry as of 2023.)
Deng W, Jo J*, Tanka T, Morikuni H, Hashimoto Y, Matsumoto N, Honda Y*. A senomorphic-conjugated scaffold for application of senescent cells in regenerative medicine. Adv Ther 2023; 6: 2200276.
(Aging cell/regenerative medicine research) This research tackled the development of bone replacement materials from the new perspective of controlling aging cells. It was revealed that the bone regeneration ability of aged pluripotent progenitor cells can be restored by using a material that combines catechin, which has the ability to control aging cells. This result will lead to effective regenerative medicine for the elderly and the effective use of mass-produced cells.
Yang N, Nakagawa M*, Nishiura A, Yamada M, Morikuni H, Honda Y*, Matsumoto N. Identification of senescent cells in peri-implantitis and prevention of mini-implant loss using senolytics. Int J Mol Sci 2023; 24: 2507.
(Senescent cell research) Peri-implantitis is a difficult disease that can cause dental implants to fall off. We created a unique rat model and reported that senescent cells appear due to the stress of peri-implantitis, and that fall-off can be prevented by using a drug that removes senescent cells. This finding will lead to the development of next-generation prevention and treatment methods for peri-implantitis.
Liu H, Gong Y, Nakagawa M, Tanimoto H, Yoshikawa K, Honda Y*, Yamamoto K. Localization of senescent cells under cavity preparations in rats and restoration of reparative dentin formation by senolytics. Dent Mater J 2023; 42: 360-367.
(Aging cell research) We have clarified that the stress caused by cavity formation in dental treatment induces senescence in dental pulp cells. We have also reported that removing these cells with a senolytic drug accelerates the formation of repair dentin. These findings could lead to the development of a new treatment for tooth decay.
Zhou Y, Nishiura A*, Morikuni H, Tsujibayashi T, Honda Y, Matsumoto N. Development of a tooth movement model of root resorption during intrusive orthodontic treatment. Dent Mater J 2023; 42: 396-404.
(Equipment development) With the help of the professors in the orthodontics department, we created a new rat model of vertical root pressure. This was the development research that became the foundational tool for the IJOS paper mentioned above.
2025
Wang X, Honda Y*, Zhao J, Morikuni H, Nishiura A, et al.: Enhancement of bone-forming ability on beta-tricalcium phosphate by modulating cellular senescence mechanisms using senolytics. Int J Mol Sci 2021, 22: 12415.
(Aging cell research) We discovered that stress-induced premature senescent cells appear around β-TCP, a bone filler used in bone regeneration, and demonstrated the possibility of enhancing bone regeneration by controlling this mechanism.
Gong Y, Honda Y*, Adachi T, Marin E, Yoshikawa K, et al.: Tailoring silicon nitride surface chemistry for facilitating odontogenic differentiation of rat dental pulp cells. Int J Mol Sci 2021, 22: 13130.
(Stem cell research) We have revealed that silicon nitride, which is rarely used in dentistry, may promote the differentiation of dental pulp-derived cells into odontoblasts.
(Bone regeneration research) We have created a β-TCP-containing gel by utilizing gelatin bound to the new bone filler epigallocatechin gallate (EGCG), and have succeeded in improving the operability, cell affinity, and dispersion inhibition of β-TCP, as well as bone regeneration ability of β-TCP.